Development of an L gene real-time reverse-transcription PCR assay for the detection of avian paramyxovirus type 1 RNA in clinical samples
Identifieur interne : 000F59 ( Main/Exploration ); précédent : 000F58; suivant : 000F60Development of an L gene real-time reverse-transcription PCR assay for the detection of avian paramyxovirus type 1 RNA in clinical samples
Auteurs : Chad M. Fuller [Royaume-Uni] ; Lina Brodd [Royaume-Uni] ; Richard M. Irvine [Royaume-Uni] ; Dennis J. Alexander [Royaume-Uni] ; Elizabeth W. Aldous [Royaume-Uni]Source :
- Archives of Virology [ 0304-8608 ] ; 2010-06-01.
Abstract
Abstract: A real-time reverse-transcription PCR (rRT-PCR) that targets a region of the polymerase (L) gene was developed to detect all known lineages of avian paramyxovirus type 1 (APMV-1), also known as Newcastle disease virus (NDV). A panel of 23 viruses representing the current known phylogenetic diversity of the APMV-1 population with a bias towards the more recent European strains, which had been grown in embryonated fowls’ eggs, were tested. A range of positive and negative clinical samples (n = 350) provided by the National Reference Laboratory and International Reference Laboratory at VLA Weybridge were also tested. Positive clinical material included samples considered representative of lineages 3, 4 and 5 obtained from chickens, ducks, pigeons and partridges. The negative sample population was obtained from chickens, turkeys and ducks. The APMV-1 L gene rRT-PCR gave high relative sensitivity (96.05%) and specificity (98.18%) when compared with virus isolation in embryonated fowls’ eggs. It is proposed that this assay could provide a first-line screening tool for the detection of APMV-1 in clinical samples.
Url:
DOI: 10.1007/s00705-010-0632-1
Affiliations:
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A panel of 23 viruses representing the current known phylogenetic diversity of the APMV-1 population with a bias towards the more recent European strains, which had been grown in embryonated fowls’ eggs, were tested. A range of positive and negative clinical samples (n = 350) provided by the National Reference Laboratory and International Reference Laboratory at VLA Weybridge were also tested. Positive clinical material included samples considered representative of lineages 3, 4 and 5 obtained from chickens, ducks, pigeons and partridges. The negative sample population was obtained from chickens, turkeys and ducks. The APMV-1 L gene rRT-PCR gave high relative sensitivity (96.05%) and specificity (98.18%) when compared with virus isolation in embryonated fowls’ eggs. 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Fuller</name><name sortKey="Irvine, Richard M" sort="Irvine, Richard M" uniqKey="Irvine R" first="Richard M." last="Irvine">Richard M. Irvine</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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